Ukr. Bioorg. Acta 2020, Vol. 15, N1, 42-46.
Investigation of the extract's composition of Viper's bugloss (Echium vulgare)
Anna R. Kapusterynska, Vira R. Hamada, Anna S. Krvavych, Roksolana T. Konechna, Maria S. Kurka, Volodymyr P. Novikov
Lviv Polytechnic National University, 79013 Lviv, Ukraine
The characteristics of Viper's bugloss (Echium vulgare) plant, its pharmacological properties, and extracts’ composition are presented in this study. Results of the literature analysis, data on the biologically active compounds and areas of use of this medicinal plant are summarized. Viper's bugloss (Echium vulgare) is a species of flowering plant in the borage family Boraginaceae. It is native to most of Europe as well as western and central Asia. Viper's bugloss (Echium vulgare) is a plant that has been utilized as food (honey), medicine, a poison, an oil, and as a dye and tannin-producing ornamental plant. Viper's bugloss (Echium vulgare) is especially rich in pyrrolizidine alkaloids, flavonoids, phenolcarboxylic acids, sterones and naphthoquinones. In traditional medicine, Viper's bugloss (Echium vulgare) is utilized as exhilarant and a mood stimulant. That is why one of the possible uses of this plant is considered to be treatment of depressive states. Like most representatives of Boraginaceae family, it has been insufficiently studied. No previous work quantifying flavonoids content of aerial parts of Viper's bugloss (Echium vulgare) growing in Ukraine has been presented. Continuing the studies of this species, the aqueous and ethanolic extracts from Viper's bugloss (Echium vulgare) aerial parts were obtained and their phytochemical composition was investigated. For the first time, the qualitative analysis of Viper’s bugloss’s extracts in relation to biologically active compounds and the quantitative analysis of flavonoids by aluminum chloride spectrophotometric method are reported. The experimental results indicated that the total flavonoid concentration was 2.59% in extract. The maximum yield of extractives was found to be 16%. The obtained research data will be used in future investigations.
Viper's bugloss (Echium vulgare); biologically active substances; flavonoids; depression.
1. Kapusterynska À. R., Hamada V. R., Krvavych À. S., Konechna R. Ò., Novikov V. P. Perspektyva vykorystannia dosvidu narodnoi medytsyny dlia likuvannia depresyvnykh ta tryvozhnykh staniv, 6th International Scientific and Practical Internet Conference “Modern Movement of Science”, Dnipro, Ukraine, 2019, 418-423 (In Ukr.).
2. State Pharmacopoeia of Ukraine. State Enterprise “Scientific-Expert Pharmacopoeia Center”, 1st ed., RIREG: Kharkiv, 2001; p 376 (In Ukr.).
3. Wikipedia contributors. Echium vulgare [Internet]. Wikipedia, The Free Encyclopedia; 2020 Jan 9, 19:38 UTC. Available from:
https://en.wikipedia.org/w/index.php?title=Echium_vulgare&oldid=93499075. (accessed on June 10, 2020).
4. Medicinal plants: Encyclopedic Reference book, Grodzinsky, A. M., Ed., UVKTS “Olimp”: Kyiv, 1992; p 397 (in Ukr.).
5. Gontova, T. M. Study of blueweed polysaccharides. Problems of ecological and medical genetics and clinical immunology 2013, 116, 132-137 (in Ukr.).
6. Ozcan, T. Analysis of the total oil and fatty acid composition of seeds of some Boraginaceae taxa from Turkey. Plant Systematics and Evolution 2008, 274. 143-153.
7. Alsanie, W. F. et al. Viper's Bugloss (Echium Vulgare L) Extract as A Natural Antioxidant and Its Effect on Hyperlipidemia. International Journal of Pharmaceutical and Phytopharmacological Research (eIJPPR) 2018, 8, 81-89.
8. Kuruuzum-Uz, A. et al. Phytochemical and antimicrobial investigation of Echium vulgare growing in Turkey. Biochemical systematics and ecology 2004, 32, 833-836.
9. Moalem, S. A.; Hosseinzadeh, H.; GhonchehIranian, F. Evaluation of Antidepressant Effects of Aerial Parts of Echium vulgare on Mice. Iran J. Basic Med. Sci. 2007, 10, 189-196.
10. Boppre, M.; Colegate, S. M.; Edgar, J. A. Pyrrolizidine alkaloids of Echium vulgare honey found in pure pollen. J. Agric. Food Chem. 2005, 53, 594-600.
11. Lee, S. T.; Schoch, T. K.; Stegelmeier, B. L.; Gardner, D. R. et al. Development of enzyme-linked immunosorbent assays for the hepatotoxic alkaloids riddelliine and riddelliine N-oxide. J. Agric. Food Chem. 2001, 49, 4144-4151.
12. Mashtaler, L. V. V. Content of amino acids in dense extracts from raw material of Echium vulgare L. Zaporozhye medical journal. 2013, 79, 43-44 (in Ukr.).
13. Gontova, T.M.; Mashtaler, V.V.; Mishneva, K.D. Investigations organic acids and vitamins in raw materials of the Symphytum and the Echium genus.Phytochemical researches 2013, 25, 44-46 (In Ukr.).