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Ukr. Bioorg. Acta 2020, Vol. 15, N1, 53-58.

Theoretical evaluation of electroanalitical determination of diazoline (mebhydrolin) on a polymer electrode

Volodymyr V. Tkach1,2, Marta V. Kushnir1, Yana G. Ivanushko1,3, Silvio C. de Oliveira2, Olga V. Luganska4, Petro I. Yagodynets1, Zholt O. Kormosh5

1 Yuriy Fedkovich Chernivtsi National University, 2 Kotsiubynsky St., Chernivtsi, 58012, Ukraine
tel.: +380-50-640-0359; e-mail: nightwatcher2401@gmail.com
2 Universidade Federal de Mato Grosso do Sul, Ave. Sen. Felinto. Muller, 1555, C/P. 549, 79074-460, Campo Grande, MS, Brazil
3 Bukovinian State Medical University, 9 Teatralna Sq., Chernivtsi, 58000, Ukraine
4 Zaporizhzhya National University, 66 Zhukovskogo St., Zaporizhzhya, 69600, Ukraine
5 Lesya Ukrainka Eastern European National University, 13 Volya Ave., Lutsk, 43025, Ukraine

ABSTRACT
Mebhydrolin, the active substance of diazoline, is a histamine H1-blocker that is possessing the anti-allergic, antipruritic, antioxidative properties as well as has the weak sedative effect. It is used to treat diseases and pathological conditions. Its long-term and excessive use leads to different side effects and complications such as granulocytopenia, neutropenia, dyscrasia, and granulocytosis. That is why the the development of effective methods for determining the concentration of this drug is actual. There are no reports to date available on the electrochemical determination of diazoline (mebhydrolin). Based on the structural characteristics of the molecule it can be concluded that it is an electroactive compound. Its oxidation can effectively occur on the conductive polymer layer. Moreover, the electrochemical behavior of the drug promises to be very interesting, as it is developed by a complicated mechanism. In this work, the electrochemical determination of a mebhydrolin concentration on the leading polymer was studied from a theoretical point of view. The polymerization and the reactions sequences was describe by a mathematical model, which was derived and analyzed using linear stability theory and bifurcation analysis. From the model analysis we concluded that: a). The polymer electrode promotes an electrooxidation of mebhydrolin and the system is electroanalytically effective. The relationship between the electrochemical parameter (the current) and the concentration of nitrite is described and it is linear in nature. Therefore, the analytical signal can be easily interpreted. b). The electroanalytical process occurs in the diffusion mode at low concentrations of the analyte and in the adsorption mode at high concentration. c). The oscillatory behavior of this system is possible. It is caused by the effects of the electrochemical stage on PES as well as also by surface instabilities.

KEYWORDS
chemically modified electrodes, mebhydrolin, conductive polymers, electrochemical sensors, steady stationary state.

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